Application |
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
Component |
Type Strain |
Volume |
Biosafety Level |
Composition |
Product Format |
Storage |
Storage Conditions |
Lysis Buffer |
梅經(jīng)理 |
17280875617 |
1438578920 |
胡經(jīng)理 |
13345964880 |
2438244627 |
周經(jīng)理 |
17757487661 |
1296385441 |
于經(jīng)理 |
18067160830 |
2088210172 |
沈經(jīng)理 |
19548299266 |
2662369050 |
李經(jīng)理 |
13626845108 |
972239479 |
中阳县|
玉门市|
固始县|
安达市|
革吉县|
建始县|
石城县|
伊吾县|
错那县|
鹤山市|
漯河市|
扬州市|
饶河县|
浠水县|
寿阳县|
讷河市|
鲁山县|
永昌县|
五莲县|
察哈|
大理市|
定安县|
桦川县|
平远县|
汨罗市|
张家川|
沙湾县|
苏州市|
丽江市|
龙海市|
孝感市|
五台县|
新建县|
安吉县|
吉首市|
华宁县|
洛阳市|
安仁县|
临朐县|
贵南县|
甘德县|
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