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Kilham rat virus
Kilham rat virus
規(guī)格:
貨期:
編號(hào):B162946
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Kilham rat virus
商品貨號(hào) B162946
Classification Parvoviridae, Parvovirus
Deposited As Deposited as Rat virus
Agent Kilham rat virus
Strain Kilham (RV-13)
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1790 images
Comments Derived by adaptation of primary cell culture product ATCC® VR-235 to NRK (ATCC® CRL-6509) cells. ATCC® VR-235 and ATCC® VR-1790have not been compared for sequence or infectivity in primary cell culture and tissue culture. The virus produces acute fatal disease, stunted growth, or latent infection in hamsters (i.c.), depending on dose of virus and age of animals. Spontaneous transmission from infected to uninoculated hamsters may occur. Virus from tissues of cell cultures will agglutinate guinea pig red blood cells at room temperature. Morphology resembles that of polyoma virus.
Effect on Host CPE, cell rounding, degeneration, cytoplasmic bridging
Recommended Host NRK (ATCC® CRL-6509™)
Growth Conditions Temperature: 37°C

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 40-60% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.

Incubation: 2-7 days
Name of Depositor L Kilham, Dartmouth Medical School
Source Tissues of tumor-bearing rats
References

Kilham L, Olivier LJ. A latent virus of rats isolated in tissue culture. Virology 7: 428-437, 1959. PubMed: 13669314

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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