99精品久久99久久久久,亚洲国产成人精品无码一区二区,国产精品亚洲综合色区韩国,久久精品第一国产久精国产宅男66,亚洲国产日韩欧美一区二区三区,久久久久久精品免费无码无,国产中年熟女高潮大集合,亚洲AV无码成H在线观看

in suitable host, produces protein modification methylase AccI

in suitable host, produces protein restriction endonuclease AccI

DNA: genomic

Insert lengths(kb): 4.199999809265137

Gene product: restriction endonuclease AccI [hsdM]

Target Gene: modification methylase AccI, restriction endonuclease AccI

Restriction digests of the clone give the following sizes (kb): XbaI/PstI--4.5, 4.0, 2.6.

The ClaI fragment (which includes pBR322 ClaI/EcoRI sequence) contains the following restriction sites (approximate kb from the vector-derived end): EcoRI--0.02, 1.8; XmnI--0.9, 3.9; HindIII--3.3, 3.5; BanII--4.1; KpnI--4.1.

To optimize production, the plasmid should be transformed into E. coli MM294, grown at 30C to late log phase, and then shifted to 42C for an additional 3 hr.

The vector is pUC19 modified by insertion of a 4.3 kb BamHI fragment containing bacteriophage lambda N, PR, cI857, and PR sequences. The PL promoter drives transcription of the insert.

Distributed in aliquots of 100 ng dried.