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Size (kb): 4.9120001792907720

DESCRIPTION OF VECTOR:
Intact vector size: 4.912
Type of vector: phagemid
Cloning sites: PstI SalI XbaI BamHI SmaI
Polylinker sites: PstI SalI XbaI BamHI SmaI
Construction: pTZ18R, bacteriophage f1, CMV promoter
Features (with orientation and position when available):
MCS: PstI...SmaI
transcription terminator: hGH
enhancer: SV40
marker(s): ampR, <-
replicon: f1, <-
promoter: CMV, ->

Vector: pCMV8 (phagemid)

Promoters: Promoter CMV

Construction: pTZ18R, bacteriophage f1, CMV promoter

Marker(s):ampR

Construct size (kb): 4.912000179290772

Features: marker(s): ampR
promoter: CMV
replicon: f1
MCS: PstI...SmaI
transcription terminator: hGH
enhancer: SV40

cloning vector

Restriction digests of the clone give the following sizes (kb): BamHI--4.9; SalI--4.9; XbaI--4.9.

Should be grown in media containing ampicillin at a final concentration of 1 mg/ml to improve plasmid yields.

May be used in essentially all cultured cells in transient transfection assays, to establish permanent cell lines and to construct transgenic mice.

The pCMV7 plasmid contains a hybrid intron, while the pCMV8 plasmid contains both a hybrid intron and a 5' untranslated segment corresponding to the adenovirus major late tripartite leader sequence.

Temperature: 37.0°C