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pmIL1AcDNA [murine IL-1alpha cDNA]
pmIL1AcDNA [murine IL-1alpha cDNA]
規(guī)格:
貨期:
編號(hào):B239577
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pmIL1AcDNA [murine IL-1alpha cDNA]
商品貨號(hào) B239577
Designations pmIL1AcDNA [murine IL-1alpha cDNA]
GenBank Number

X01450

Species Mus musculus, mouse
Depositors U Gubler
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 2.0
Tissue: P388D1 cell line
Gene product: interleukin 1, alpha [Il1a]
Insert Size (kb) 2.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt positions according to the published sequence): HpaI--1188; PstI--373; PvuII--789; ScaI--1111; XbaI--1585.
Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--3.0, 2.0; PstI--5.0; PvuII--2.7, 1.5, 0.8; EcoRI--uncut; XbaI--4.7, 0.2.
The BstNI site at nt 8 was filled in and a HindIII linker was added. The 3' end of the insert includes the poly(A) tail, the added GC tail, and the BamHI site engineered from the original pMG-5 vector.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Lomedico PT, et al. Cloning and expression of murine interleukin-1 cDNA in Escherichia coli. Nature 312: 458-462, 1984. PubMed: 6209582

Ueli Gubler, personal communication

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