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pTC191
pTC191
規(guī)格:
貨期:
編號(hào):B240196
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pTC191
商品貨號(hào) B240196
Designations pTC191
Depositors A Talavera
Biosafety Level 1
Vector Information
Size (kb): 2.7000000476837160
Vector: pTC191 (plasmid)
Construction: pUC19
Marker(s):ampR
Construct size (kb): 2.700000047683716
Features: insert detection: lacZ'
marker(s): ampR
replicon: pMB1
MCS: BamHI...HindIII
Applications
vector containing primer sites useful for sequencing
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.15; NcoI--2.7; PvuII--2.3, 0.40.
The plasmid contains the following restriction sites (approximate kb from nt 1): BglI--0.24, 1.85; NcoI--0.45; PvuI--0.28, 2.10; PvuII--0.31, 0.67.
Expression vector designed for converting restriction sites at the end of inserts. Also permits insert detection by lacZalpha complementation and sequencing from M13 universal and reverse primers.
Constructed from pUC19 by modification of the multiple cloning site to include some unique cloning sites surrounded by some repeated sites.
Pairs of identical restriction sites are in the same reading frame to maintain lacZ' insert detection regardless of the enzyme used.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

DeLaFuente A, et al. Restriction end-converting vectors with tandem repeated multiple cloning sites. Gene 139: 83-86, 1994. PubMed: 8112593

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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