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lambda2 [GC.2]
lambda2 [GC.2]
規(guī)格:
貨期:
編號(hào):B242797
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 lambda2 [GC.2]
商品貨號(hào) B242797
Designations lambda2 [GC.2]
GenBank Number

L10641

Species Homo sapiens, human
Depositors A Dugaiczyk
Vector
Construct size (kb): 49.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 18.0
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-18000 of the insert correspond to
nucleotides ~37000-55136 of L10641.
Insert lengths(kb): 18.0
Tissue: fetal liver (ATCC 37333)
Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]
Insert Size (kb) 18.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 9.8, 3.5, 3.3, 1.4; HindIII--19, 14, 6, 4.4, 4, 2, 0.4; BamHI--17, 11, 7.5, 5.8, 3.9, 3, 1.6, 0.5; BglII-->23, 5.8, 5, 2.15, 1.95, 1.9, 1.8, 1.4, 0.7, 0.4;
SstI-->23, 15, 4, 3.8, 1.9.
The insert contains the following restriction site (approximate kb from the 5' end): EcoRI--1.4, 4.7, 14.5; HindIII--6.6, 11.0, 15.0, 15.4, 17.4; SacI--14.6, 18.8.
Begins in intron 11 and extends into the 3' flanking region.
ATCC 95668/95669 and 95670/95671 overlap by 11.9 kb and share an EcoRI fragment of 3.3 kb.
Contains Alu repeats, L1 (Kpn) repeats, and a THE repeat.
References

Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650

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