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pYNH24
pYNH24
規(guī)格:
貨期:
編號(hào):B243444
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pYNH24
商品貨號(hào) B243444
Designations pYNH24
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Vector
Construct size (kb): 4.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 2.0
Gene product: DNA Segment, single copy [D2S44]
Insert Size (kb) 2.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; HindIII--4.6; EcoRI/HindIII--2.5, 1.95; PvuII--2.15, 1.53, 0.76; BglI--3.6, 1.08.
MspI fragment from cYNH24 cloned into AccI site of pUC18; RFLPs detected under normal hybridization stringency; washes at 0.1X SSC, 0.1% SDS, 65 C.
Enzyme(s) not detecting polymorphism: RsaI.
References

Odelberg SJ, et al. Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5: 915-924, 1989. PubMed: 2574152

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pYNH24 on chromosome 2 (D2S44). Nucleic Acids Res. 15: 10073, 1987. PubMed: 2892167

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